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cailynn johnson
G-banding karyotype analysis is essential for detecting chromosomal abnormalities in iPSC cultures. This technique stains metaphase chromosomes to reveal banding patterns, enabling identification of aneuploidies, translocations, deletions, and duplications. Regular karyotyping every 10–15 passages ensures genomic integrity, critical for therapeutic applications.
cailynn johnson
Embryoid body formation assay is a key method for evaluating iPSC pluripotency and germ layer differentiation. By culturing iPSCs in suspension, researchers induce spontaneous aggregation into 3D structures representing ectoderm, mesoderm, and endoderm. Molecular and histological analyses confirm lineage-specific marker expression, supporting studies in regenerative medicine and disease modeling.
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cailynn johnson
Immortalized cell lines are genetically modified cells capable of indefinite proliferation, making them ideal for long-term and high-throughput experiments. Unlike primary cells, which closely mimic in vivo conditions but have limited lifespan, immortalized lines offer consistency, scalability, and ease of cultivation.
cailynn johnson
iPSC-derived blood-brain barrier (BBB) models replicate human BBB structure using stem cell-derived endothelial cells, astrocytes, and pericytes. These models offer high human specificity and customizable features for drug screening, disease modeling, and permeability prediction.
cailynn johnson
Brain spheroids are 3D cellular models derived from human stem cells that mimic the structure and function of the brain. They include neurons, astrocytes, and endothelial cells, forming a microenvironment that replicates blood-brain barrier (BBB) properties.
cailynn johnson
Neural differentiation involves guiding pluripotent stem cells to become specific neural cell types such as neurons, astrocytes, and oligodendrocytes. This process uses signaling molecules like retinoic acid and BMP to activate neural genes and form neural rosettes.
cailynn johnson
Clier-qPCR is a sensitive method for validating and quantifying glycosylated RNAs using click chemistry and real-time PCR. It enriches glycoRNAs through biotin labeling and magnetic bead capture, followed by ΔΔCt-based quantification.
cailynn johnson
GlycoRNA imaging enables spatial visualization of glycosylated RNAs at the single-cell level. Using dual-recognition probes and signal amplification, this technique detects glycoRNAs on cell surfaces and within subcellular structures.
cailynn johnson
GlycoRNA-seq is a specialized sequencing method designed to detect and analyze glycosylated small noncoding RNAs. Unlike traditional RNA-seq, it enriches for RNAs modified with glycans—especially those containing sialic acid and fucose—using techniques like metabolic labeling and click chemistry.
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